The CD44 antibody is a cell-surface glycoprotein that is expressed on monocytes, lymphocytes, and granulocytes. It can also be a homing cell adhesion molecule. It has been implicated in lymphocyte homing, tumor metastasis, and cell migration. It is designed to be used for research only. It does have a clone called SPM 521, and the immunogen is the synthetic peptide that corresponds to the human’s CD44. The isotype is the IgG2a. It has no known epitope and a molecular weight of 80kDa.
Applications
The CD44 antibody is designed to be used with Immunohistochemistry and Western Blotting applications.
For IHC applications, you should use formalin-fixed or paraffin-embedded tissues. Likewise, deparaffinized slides should be used, as well. You’ll find a pre-diluted formula, as well as three sizes of concentrated versions. When using the concentrated format, you should dilute the antibody with a ratio of 1:50.
To retrieve the antigen, you should boil tissue sections in a 10mM citrate buffer with a pH of 6.0. This should be done for 10 minutes, allowing the concoction to cool to room temperature for 20 minutes. Likewise, it should also be incubated for 30 minutes while at room temperature. The positive control is the esophageal carcinoma.
For Western Blotting, you should dilute the antibody using a ratio of 1:25, allowing it to incubate for one hour at room temperature. It isn’t recommended to use the pre-diluted formula for Western Blotting because the pre-diluted formula is diluted using a ratio of 1:50 for IHC purposes. Likewise, the positive control is the HeLa cell lysate with cellular localization occurring in the membrane.
The CD44 antibody can help you learn more about lymphocytes, tumors, and other things. Visit Spring Bioscience now to learn more about the product, how to purchase it, and the formulations available.