Find Out More About The BRAF Antibody
The BRAF antibody (serine/threonine-protein kinase B-raf) is part of the Raf family. It is used in the transduction of signals to the nucleus from the cell membrane. Mutations are frequent in both benign and malignant tumors, including papillary thyroid carcinoma, ovarian cancer, melanoma, and colorectal carcinoma.
More About The BRAF Monoclonal Antibody
This product is designed for research purposes and is not to be used in diagnostic procedures. Its clone is called pBR1, and it has a molecular weight of 95 kDa. It has been tested on humans. The applications for this product include immunohistochemistry, IHC, or Western Blotting, which is recommended by untested.
The IHC procedure involves preparing the specimen using Paraffin-embedded, formalin-fixed tissues. You’ll also need to deparaffinize the slides, dilute the product, retrieve the antigens, and consider incubation periods carefully.
The positive control for IHC procedures is colon carcinoma with a cellular localization in the cytoplasm. It is recommended to store this product between two and eight degrees Celsius.
More Information About The Mutation V600e
The mutation of the BRAF antibody is the V600E, which accounts for most of the alternations. The mutation can induce a conformational change in the activation segment, which leads to constitutive kinase activity. It has been detected in papillary thyroid carcinoma, melanoma, borderline ovarian cancer, pleomorphic xanthoastrocytomas, ganglioglioma, and more.
It also has a clone called VE1 and has a molecular weight of 95 kDa. Its application is for immunohistochemistry (IHC) only.
It comes in a 0.1 ml, 0.5 ml, and 1.0 ml version, all of which use a Tris buffer pH of 7.5 and a 0.3 percent carrier protein with less than 0.1 percent sodium azide.
The BRAF antibody is available from Spring Bioscience. Visit them now at www.springbio.com to learn more about this product and what forms it currently comes in before purchasing now.